I downloaded Windows Process Explorer and searched for any processes using the .Rmd file (there were none). I then looked for the project directory and found a process for rsession.exe with .Rproj.user\B12BF9AEF\source\s-4121B91\lock_file. There were another two processes for rsession.exe with the same PID, one process for rstudio.exe and one for explorer.exe .
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